Journal: Diabetologia

Article Title: Enhanced VEGF signalling mediates cerebral neovascularisation via downregulation of guidance protein ROBO4 in a rat model of diabetes

doi: 10.1007/s00125-017-4214-6

Figure Lengend Snippet: ROBO4 overexpression increases vascular stability. (a) Nuclear morphology was used to assess the pericyte-to-endothelial cell ratio. Dotted lines represent the elliptical endothelial cells nuclei and solid circles represent pericytes nuclei. Overexpression of ROBO4 (Robo4 AD) significantly increased this ratio in the cerebral vasculature (n=4, *p<0.05 vs contralateral side). Scale bars, 10 μm. (b) Representative images of brain sections showing FITC-filled vessels (green), nuclear stain (DAPI, blue), pericyte marker, PDGF receptor-β antibody (red) and merged images. Scale bars, 10 μm

Article Snippet: Sections were reacted to rabbit polyclonal anti-platelet-derived growth factor (PDGF) receptor-β antibody (Santa Cruz, Cambridge, MA, USA).

Techniques: Over Expression, Staining, Marker

Journal: Oncology Letters

Article Title: Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium

doi: 10.3892/ol.2016.4690

Figure Lengend Snippet: DiI-retaining cells possess multipotency in vitro . (A) Expression of GFAP, βIII-tubulin and PDGFRα in DiI-retaining and DiI-negative cells in the NCH421k cell line prior to and following differentiation as assayed by western blot, with β-actin as the internal reference. Quantification of the band densities of (B-a) GFAP, (B-b) βIII-tubulin and (B-c) PDGFRα through normalization to β-actin using ImageJ software. The data are presented as the mean ± standard deviation from three independent experiments. *P<0.05 compared with expression prior to differentiation in the DiI-retaining group. GFAP, glial fibrillary acidic protein; PDGFRα, platelet-derived growth factor receptor α.

Article Snippet: The polyvinylidene difluoride membrane was washed with TST buffer (G-Biosciences) and subsequently incubated with the following primary antibodies at 4°C overnight: Mouse monoclonal anti-human glial fibrillary acidic protein (GFAP; dilution, 1:10; catalog no., MA1045; Boster Systems, Inc.), rabbit polyclonal anti-human platelet-derived growth factor receptor α (PDGFRα; dilution, 1:10; catalog no., PA1678; Boster Systems, Inc.) or mouse monoclonal anti-human βIII-tubulin (dilution, 1:10; catalog no., MA1112; Boster Systems, Inc.).

Techniques: In Vitro, Expressing, Western Blot, Software, Standard Deviation, Derivative Assay